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On Monday, DNR biologists searched areas of Dunkard Creek for any remaining live fish.
Last month dead fish, mussels and salamanders started appearing on the creek banks. Since then thousands of fish and the streams entire mussel population have died.
The West Virginia DEP says golden algae was the main culprit, but the agency continues to investigate the role mining activities might have played.
Mine drainage from Consol's Blacksville coal mine contained high levels of chloride, which environmental officials in Pennsylvania say caused the golden algae to spread and kill the aquatic life.
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